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A simple isoelectric focusing technique is presented for the screening of α- and β-globin chain variants on polyacrylamide gel in the presence of 8 M urea. No detergent was used. Variants focused at discrete pH values, depending on the charge variation (-2, -1, 0, +1 or +2) introduced by the...
A hemoglobinopathy survey totaling 22, 921 specimens was conducted for the past three years in the Takamatsu district. The results were reviewed comprehensively from the aspect of population genetics. Six abnormal hemoglobins were discovered in eighteen families including eleven families with Hb...
Separation of the globin polypeptide chains of hemoglobin using electrophoresis on polyacrylamide gels in acid, urea, and Triton X-100 was initially described by Rovera et al (1). We have utilized this method for human (2) and murine systems (3), and have shown that the technique will separate...
Hb Oleander (α116(GH4)Glun→Gln) was found in a newborn black infant and his mother during a screening program. Electrophoretically, Hb Oleander moves like Hb S on cellulose acetate, pH 8.5 and like Hb A on citrate agar, pH 6.0. The abnormal α chain moves cathodically to α G Philadelphia...
Microcytic red cells from a 70 year old Negro man with mild anemia contained only hemoglobin G-Philadelphia. Red cells from all of his children had low-normal MCV's, and contained 32-34 percent of the abnormal hemoglobin. Oxygen affinity of his blood and stability of his hemolysate were normal,...
This report describes a new abnormal hemoglobin discovered in a 43-year-old Japanese female living in Machida City, Tokyo Capital City, in October 1981. Hematological study of the propositus, who was clinically healthy, showed neither microcytic hypochromic anemia due to iron deficiency, nor...
The oxygen binding properties of Hb solutions ‘stripped’ of phosphates were studied in seven different 50 mM buffer systems. Hb-oxygen affinity was higher in HEPES and HEPPS buffers than in bis-tris, bis-tris propane, imidazole, MOPS and tris buffers below pH 7.4 in the presence of 100 mM...
A silent mutant of the β chain of the baboon ( Papio cynocephalus ) was detected by high performance liquid chromatography of the globin chains. The substitution is β13 Ala → Thr. The inclusion of nonylamine in the developers for HPLC results not only in an improved separation of baboon...
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