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High pressure liquid chromatography (HPLC) has been used for the detection and quantitation of the β chain variants Hb S and Hb C in blood samples of newborn babies with different hemoglobinopathies. The complete separation of the Hbs C, S, A, and F made it possible to diagnose conditions such...
Population surveys performed in different Brazilian ethnic groups, and comprising 23,606 subjects, disclosed, besides the common S and D types, hemoglobins G and Po: to Alegre, as well as high F, A' 2 and an apparently unstable A 2 variant. Additional studies on isolated families revealed the...
We describe a rapid procedure for constructing cloned human genomic libraries from small amounts of peripheral blood. High molecular weight DNA is isolated from 5–20 ml peripheral blood, partially cleaved with Eco R1, and 8–22 kb fragments are cloned using bacteriophage Charon 4A and a...
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