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According to the Chinese calendar 2008 is the Year of the Rat/Mouse.
The sciarid DNA puff C4 BhC4‐1 gene is amplified and transcribed in salivary glands at the end of the larval stage. In transgenic Drosophila, the BhC4‐1 promoter drives transcription in prepupal salivary glands and in the ring gland of late embryos. A bioinformatics analysis has identified...
Retinoids play critical regulatory roles in the maintenance of mammalian epithelia and exert pleiotropic effects through nuclear receptors. RXRα, which is a ligand‐dependent transcription factor, is the most abundant RXR isotype expressed in cervical epithelia, and may play a crucial role in...
Heparan sulfate (HS) proteoglycans modulate the biological activity of a number of growth factors in development, homeostasis, and cancer. Specific modifications of HS chains by HS biosynthetic enzymes have been implicated in growth factor signaling in multiple aspects of organogenesis. Although...
Despite the broad use of morpholino antisense oligonucleotides (MO) to knockdown gene function in zebrafish embryos, the efficiency of this method has not been successfully assessed, particularly in the cases of translation‐blocking MOs. In our current study, we describe a luciferase...
Six known proteins bind to the insulin‐like growth factor (IGF) with high affinity. Igfbp5 encodes one of these proteins, which regulates the activity of IGF, but also exerts IGF‐independent actions. Using in situ hybridization to detect cells expressing Igfbp5 mRNA, we show that Igfbp5 is...
Tissue‐specific expression of the Cre recombinase is a well‐established genetic tool to analyze gene function in specific tissues and cell types. In this report, we describe the generation of a new transgenic line that expresses Cre under the control of the rat growth hormone releasing...
We describe here a transgenic mouse line MHB‐Cre, which expresses Cre recombinase in a group of cells at the midbrain–hindbrain boundary. Using this mouse line, we studied the contribution of the boundary cells to distinct brain areas during development. Initially, the MHB‐Cre expression...
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