Genes to Cells Volume 23 Issue 3

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2018 Genes to Cells

doi:10.1111/gtc.12525

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Lem2 is retained at the nuclear envelope through its interaction with Bqt4 in fission yeast

Hirano, Yasuhiro; Kinugasa, Yasuha; Asakawa, Haruhiko; Chikashige, Yuji; Obuse, Chikashi;

2018 Genes to Cells

doi:10.1111/gtc.12557 pmid:29292846

Inner nuclear membrane (INM) proteins are thought to play important roles in modulating nuclear organization and function through their interactions with chromatin. However, these INM proteins share redundant functions in metazoans that pose difficulties for functional studies. The fission yeast...

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Transgenic mice specifically expressing amphiregulin in white adipose tissue showed less adipose tissue mass

Yang, Bo; Kumoto, Takahiro; Arima, Takeshi; Nakamura, Minako; Sanada, Yohei;

2018 Genes to Cells

doi:10.1111/gtc.12558 pmid:29341420

To determine adipocytokines that play a regulatory role during obesity development, we explored the genes that encode growth factors and investigated the physiological functions for adipose tissue development. Here, we isolated amphiregulin (Areg) gene whose expression was significantly...

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Preferable in vitro condition for maintaining faithful DNA methylation imprinting in mouse embryonic stem cells

Lee, Jiyoung; Matsuzawa, Ayumi; Shiura, Hirosuke; Sutani, Akito; Ishino, Fumitoshi

2018 Genes to Cells

doi:10.1111/gtc.12560 pmid:29356242

Epigenetic properties of cultured embryonic stem cells (ESCs), including DNA methylation imprinting, are important because they affect the developmental potential. Here, we tested a variety of culture media, including knockout serum replacement (KSR) and fetal bovine serum (FBS) with or without...

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Tumor necrosis factor‐α regulates human follicular dendritic cell‐secreted protein gene transcription in gingival epithelial cells

Iwai, Yasunobu; Noda, Keisuke; Yamazaki, Mizuho; Kato, Ayako; Mezawa, Masaru;

2018 Genes to Cells

doi:10.1111/gtc.12561 pmid:29356241

Follicular dendritic cell‐secreted protein (FDC‐SP) is a secreted protein expressed in follicular dendritic cells, periodontal ligament and junctional epithelium. To elucidate the transcriptional regulation of the human FDC‐SP gene by tumor necrosis factor‐α (TNF‐α), we conducted real‐time PCR,...

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RBMX is a component of the centromere noncoding RNP complex involved in cohesion regulation

Cho, Yukiko; Ideue, Takashi; Nagayama, Megumi; Araki, Norie; Tani, Tokio

2018 Genes to Cells

doi:10.1111/gtc.12562 pmid:29383807

Satellite I RNA, a noncoding (nc)RNA transcribed from repetitive regions in human centromeres, binds to Aurora kinase B and forms a ncRNP complex required for chromosome segregation. To examine its function in this process, we purified satellite I ncRNP complex from nuclear extracts prepared from...

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Requirement of the F‐actin‐binding activity of l‐afadin for enhancing the formation of adherens and tight junctions

Sakakibara, Shotaro; Maruo, Tomohiko; Miyata, Muneaki; Mizutani, Kiyohito; Takai, Yoshimi

2018 Genes to Cells

doi:10.1111/gtc.12566 pmid:29431241

The apical junctional complex consists of adherens junctions (AJs) and tight junctions (TJs) in polarized epithelial cells, which are attached to each other to form a sheet. Actin filaments (F‐actin) are associated with AJs and TJs and required for the formation and maintenance of this complex....

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Mutations at multiple CDK phosphorylation consensus sites on Cdt2 increase the affinity of CRL4Cdt2 for PCNA and its ubiquitination activity in S phase

Nukina, Kohei; Hayashi, Akiyo; Shiomi, Yasushi; Sugasawa, Kaoru; Ohtsubo, Motoaki;

2018 Genes to Cells

doi:10.1111/gtc.12563 pmid:29424068

CRL4Cdt2 ubiquitin ligase plays an important role maintaining genome integrity during the cell cycle. A recent report suggested that Cdk1 negatively regulates CRL4Cdt2 activity through phosphorylation of its receptor, Cdt2, but the involvement of phosphorylation remains unclear. To address this,...

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Roles of the third Ig‐like domain of Necl‐5/PVR and the fifth Ig‐like domain of the PDGF receptor in its signaling

Ueda, Yuki; Kedashiro, Shin; Maruoka, Masahiro; Mizutani, Kiyohito; Takai, Yoshimi

2018 Genes to Cells

doi:10.1111/gtc.12564 pmid:29431243

The immunoglobulin (Ig)‐like cell adhesion molecule nectin‐like molecule (Necl)‐5/poliovirus receptor is up‐regulated in many types of cancer cells and implicated in their abnormally enhanced cell proliferation and movement. We previously showed that Necl‐5 cis‐interacts with the platelet‐derived...

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MicroRNA‐664a‐5p promotes neuronal differentiation of SH‐SY5Y cells

Watanabe, Kazunori; Yamaji, Ryuhei; Ohtsuki, Takashi

2018 Genes to Cells

doi:10.1111/gtc.12559 pmid:29341475

MicroRNAs (miRNAs) belong to a class of small noncoding RNAs that play important roles in the translational regulation of gene expression. A number of miRNAs are known to act as key regulators of diverse processes such as neuronal differentiation. In this study, we have attempted to identify...

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Genes to Cells Volume 23 Issue 3

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