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Activins are dimeric proteins consisting of two inhibin β subunits. Homo- and hetero-dimerizations of two isoforms of β subunits, βA and βB, produce three forms of activins, activin-A, -B, and -AB. Recent studies have suggested that activin-A mediates gonadotropin-induced oocyte maturation in...
A pleuronectiform fish, the barfin flounder Verasper moseri , reared in a white tank had a smaller ratio of pigmented area of the skin on non-eyed side, grew faster, and had greater melanin-concentrating hormone (MCH)-immunoreactive cell bodies and MCH gene expression in the brain than in the...
Ouabain, a cardiac glycoside and inhibitor of Na + , K + -ATPase, is now believed to be a steroid hormone in mammals. We have recently identified ouabain immunoreactivity in the plasma of the tilapia, a euryhaline teleost. Changes in plasma concentrations of immunoreactive ouabain (20–40 pM) in...
Follicle-stimulating hormone (FSH) was purified, for the first time, from immature Japanese eel, Anguilla japonica , and its biochemical properties were investigated. FSH was extracted from immature eel pituitaries and purified by gel-filtration on Sephadex G-100, and two step anion-exchange...
We assessed the inhibitory effects of butyrate on the growth hormone (GH) secretion in order to investigate the cellular mechanisms in rat somatotrophs. Isolated anterior pituitary cells were cultured in DMEM for several hours, either in the presence (1, 3, or 10 mM) or absence of butyrate, and...
Two distinct yolk proteins (YP1 and YP2) were purified from the ovary of medaka, and specific antisera against YPs were generated to characterize YPs and reveal their relation to two vitellogenins (Vg1 and Vg2). The molecular masses of purified YP1 and YP2 on gel filtration were 270 and 380 kDa,...
The cDNAs encoding β-subunits of follicle-stimulating hormone (FSH) and luteinizing hormone (LH) have been cloned from the pituitary of snakehead fish, Channa maculata , and the three-dimensional structural models of the encoded FSH and LH were investigated. The cloned cDNAs, including...
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