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Our hypothesis that proline and histidine are major sites of damage during radical attack upon proteins, becoming respectively glutamate and aspartate. was investigated using proteins biosynthetically labelled with radioactive proline or histidine as targets. Free radicals were generated by...
Viable Neisseria gonorrhoeae exposed to streptonigrin generate intracellular hydroxyl radical detected by spin-trapping with 5,5-dimethyl-l-pyrroline-N-oxide; gonococci exposed to paraquat generate primarily superoxide ( J. Biol. Chem ., 262: 13404–143048, 1987). The use of streptonigrin and...
Perspectives on Mammalian Cell Death. E.d. CS Potten (1987) Oxford University Press ISBN 0–19–854184–8 £40 Chemiluminescence. Principles and Applications in Biology and Medicine. Ellis Horwood, Chichester (England); VCH Verlagsgesellschaft, Weinheim (FRG) 1988 ISSN 0930–3367; ISBN...
In mice subjected to 3-day periods of food deprivation an increase in plasma free fatty acids occurred together with a rise in the cardiac content of fatty acyl CoA-oxidase (+ 15.2%) and catalase (+ 136.2%)activities. Stimulation of hydrogen peroxide production by the heart was found after 30...
Recent investigations have indicated the presence of a fatty acid binding protein (FABP) in mammalian heart. This protein binds free fatty acids and their esters with high affinity, however, its physiological role remains unknown. Since FABP constitutes a significant amount of cystolic protein,...
CuZn superoxide dismutase levels were found to be high in developing human kidney and liver compared to some other tissues including lung. In kidney, the enzyme was expressed in proximal and distal tubules, loop of Henle and collecting tubules and after 35 weeks of gestation it appeared to be...
An electrochemical sensor for the measurement of the superoxide anion produced as a result of the respiratory burst of neutrophils is described. The reduction of cytochrome c by the superoxide ion was monitored using a surface-modified gold electrode. The current produced was shown to be...
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