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In order to overcome a common problem in spin trapping with high concentrations of 5.5-dimethyl-I-pyrroline-N-oxide (DMPO) where ESR spectra are obtained which include an unidentified set of lines composed of a triplet of doublets. commercial DMPO was analyzed for its impurities by...
The kinetics of the oxidation of human low densit) lipoprotein (LDL) can be measured continuously by monitoring the change of the 234 nm diene absorption. The time-course shows three consecutive phases, a lag-phase during which the diene absorption increases only weakly. a propagation phase with...
Electrochemical studies on metronidazole using mixed aqueous/dimethylformamide (DMF) solvents have allowed us to generate the one-electron addition product, the nitro radical anion, RNO −− 2 . Cyclic volt-ammetric techniques have been employed to study the tendency of RNO −− 2 to undergo...
Methionine was oxidized to ethylene by an ““Iron Redox”” system containing H 2 O 2 , Fe-EDTA and ascorbate. generating hydroxyl radicals or another species of similar reactivity. Oxy or met forms of haemoglobin and myoglobin were found to inhibit methionine oxidation. Methionine oxidation...
Escherichia coli lethality by hydrogen peroxide is characterized by two modes of killing. In this paper we have found that hydroxyl radicals (OH -) generated by H 2 O 2 and intracellular divalent iron are not involved in the induction of mode one lethality (i.e. cell killing produced by...
Haemorrhagic shock was produced in anaesthetized dogs, by rapid arterial bleeding to mean arterial blood pressure 35 mmHg, and maintained oligaemic for 4 h followed by return of withdrawn blood(R0WB). Dogs were observed for 72 h after ROWB for survival and recovery, and, for histopathological...
Conjugated bilirubin at low micromolar concentrations strongly inhibits the luminol-enhanced chemilu-minescence response of stimulated human polymorphonuclear leukocytes. In contrast, it does not inhibit either reduction of ferricytochrome c or lucigenin-mediated chemilum-nescence of stimulated...
The antioxidant capacity of 5-hydroxy-tryptophan. 5-hydroxy-indole. and DOPA (3,4-dihydroxy-phenyI-alanine) was tested in the Fe-induced lipid peroxidation of liver microsomes of normal- and vitamin E-deficient rats, using ascorbate as a reductant. Lipid peroxidation was monitored as low-level...
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