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Ferritin is an iron storage protein found in most living organisms. Human heavy chain (H-) and light chain (L-) ferritin were amplified from human heart cDNA library. Each ferritin gene was inserted down stream of the T7 promoter of bacterial expression, and finally four types of H-, L-, and...
A unique DNA isolation protocol based on microwave irradiation and density centrifugation for the simultaneous detection of porcine, chicken, and mutton meat species in food by PCR using newly developed primers is described. Emphasis is placed on the detection of pork, as it is considered...
Stevia reabudiana is gaining popularity especially in making diabetic food supplements in view of its non-nutritive glycosides. Steviosides profile in ex vitro and in vitro leaves and callus cultures of S. rebaudiana were investigated by HPLC analysis and further confirmed by MS using mass...
Plesiomonas shigelloides is a unique Gram-negative polarly flagellated pathogenic bacterium native to aquatic animals and environments. The genus Plesiomonas consists of a single homogeneous species. Its metabolism is similar to that of the genus Vibrio in that sugars are fermented with acid...
One hundred and three Fusarium isolates from maize samples collected from different districts of Karnataka state, India, were analyzed with genus-specific, species-specific, and potential fumonisin specific oligonucleotide primers. One set of genus-specific primers ITS F and ITS R based on a...
The aim of this study was to verify the effects of gamma radiation process on the fungal DNA and the application of PCR in the detection of Aspergillus flavus in irradiated maize grains. The samples were inoculated with a toxigenic strain and incubated under controlled conditions of relative...
Lactic acid production parameter optimization was performed using cane molasses by design of experiment (DOE) with the help of Qualitek-4 software with bigger is better as quality characteristics with eight media components at three levels in submerged culture condition. Eight factors with three...
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