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Tracheobronchial clearance was studied twice in 16 patients with chronic obstructive bronchitis after inhalation of 6 μm (aerodynamic diameter) monodisperse Teflon particles labeled with 111 In. At one exposure the particles were inhaled at an extremely slow flow, 0.05 L/s; at the other they...
The A549 cell line was used to model in vitro the interaction of alveolar epithelium with environmental particulates. Confocal and electron microscopy demonstrated A549 binding and internalization of titanium dioxide (TiO 2 ), iron oxide (Fe 2 O 3 ), concentrated ambient air particulates (CAPs),...
Using a retroviral vector expressing the adenoviral 12S E1A gene product, the authors have immortalized rat type II alveolar epithelial cells. For a period of time, the immortalized cells retain many of the ultrastructural characteristics of type II cells in situ, including the presence of...
The effects of urban air and diesel particles on inflammatory cytokine gene expression, tumor necrosis factor α (TNF-α) in particular, were studied in rat alveolar macrophages. TNF-α, interleukin (IL)-1, IL-6, cytokine-induced neutrophil chemoattractanl (CINC), and macrophage inflammatory...
Particulate and other pollutant exposures are associated with lung injury and inflammation. The purpose of this study was to develop an approach by which intact RNA could be obtained from inflamed lung tissue from particulate-exposed animals in order to correlate injury with specific gene...
This study examined the production of stored mucosubstances in rats after repeated exposure to aerosolized endotoxin, a common contaminant of bioaerosols. Male Fischer 344 rats were exposed to aerosolized saline (sham control) or endoloxin (target concentrations of 0.05, 0.5, and 5.0 μg/m 3 )...
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