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The role of collagen in pulmonary epithelial cell division and differentiation during lung development was investigated by injecting the proline analogue cis-hydroxy-L-proline (cis-HYP) to timed pregnant rats. Lung weight, DNA and 3 H thymidine incorporation were unchanged when compared to...
The effect of opioid peptide hormones on respiratory glycoprotein secretion was investigated in a cat tracheal organ culture system. Dynorphin A (dyn, 10 −5 M), alpha-endorphin (αE, 10 −5 M) and morphine sulfate (MS, 10 −4 M) stimulated mucous glycoprotein (MGP) release from airways,...
The effect of the cyclooxygenase inhibitors ibuprofen and meclofenamate were studied to assess the role of prostaglandin release in mediating the hemodynamic response to acute pulmonary microembolism in awake rabbits. In Group I (n = 10), a control group receiving only saline infusion, there was...
In our studies on activation of the Clara cell by biological substances and its relationship to pulmonary carcinogenesis, we found that large doses of glycerol induced drastic morphologic changes selectively in the Clara cell among distal airway epithelial cells in ddY mice. Subcutaneous...
Regional deposition in the lung of 111 In-labelled Teflon particles with an aerody-namic diameter of 2.5 μm was studied in eight healthy nonsmokers. The particles were inhaled at 0.5 l/sec with maximally deep breaths. Bronchoconstriction was induced by inhalation of a methacholine bromide...
An experimental approach was examined to study the growth and differentiation potential of different epithelial cell types isolated from the airways of adult rabbits. Clara cells isolated from the lungs of rabbits and a mixed cell population obtained from rabbit tracheas were injected...
A quantitative assay for the adhesion of rat lung epithelial cells has been developed. Cells are adhered to 96 well tissue culture plates and quantitated by their reaction with monoclonal antibodies to cytokeratin in an enzyme linked assay. Using this technique we have shown that rat lung...
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