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Uninjured mitotically inactive tracheal epithelium has virtually no gap junctions. When the epithelium is stimulated to proliferate, synchronously through a single wave of DNA synthesis and cell division, study of thin sections and freeze-fracture replicas by conventional transmission electron...
Lamellar bodies, the intracellular storage form of pulmonary surfactant, were isolated from adult human lung tissue. As shown by electron microscopy, the isolated human lamellar bodies resembled the lamellar bodies isolated from experimental animals. Chemical analysis revealed that the lamellar...
The properties of the phosphatidylglycerophosphate phosphohydrolase activity in lamellar bodies from rat lung have been compared with the properties of the activities responsible for the degradation of aqueously-dispersed phosphatidic acid (PA aq ) and membrane-bound phosphatidic acid (PA mb )....
Phosphatidic acid phosphatase (PAPase). which catalyzes the conversion of phosphatidic acid to 1,2-diacyl-sn-glycerol. was studied in fetal, neonatal, and adult rat lung microsomal fractions from whole lung under normal and altered physiologic states. The maximal activity was obtained at pH 7.0...
Bacterial lipopolysaccharide (LPS) stimulates pulmonary macrophages from BCG immune-rechallenged hamsters to kill tumor cells in vitro. However, pulmonary macrophages from BCG immune and from untreated hamsters cannot be activated for tumor cytotoxicity by in vitro treatment with LPS. Pulmonary...
In order to study specific anatomical relationships among organelles involved in the movement and secretion of mucin granules by airway epithelium, a three-dimensional replica of a single goblet cell from porcine trachea was constructed by means of transmission electron microscopy of serially...
Systemic vascular byporesponsiveness has been well documented in aged rats, but a similar decreased vasoreactivity of the pulmonary vasculature has not been reported. Isolated lungs from young (3–5 months) and middle-aged (12–14 months) rats were perfused with whole blood and challenged...
Oral administration of 1.1-dichloroethylene (1.1-DCE) produces acute injury to the lungs of C57B1/6 mice. The bronchiolar epithelium is most severely affected with damage selective for Clara cells. After a 100 mg kg/dose of 1.1-DCE. Clara cells show extensive dilatation of cisternae and...
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