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We examined the ability of four commonly used culture media to support prolactin (PRL), growth hormone (GH), and adrenocorticotropic hormone (ACTH) release, as well as the inhibitory PRL response to dopamine. After a week of primary culture, rat anterior pituitary cells from both genders were...
Chloroquine increased the binding of 125 I-labeled insulin to rat hepatoma cells (R-Y121B) at 4°C. The effect of chloroquine on insulin binding was amplified at 23°C, and a large increase in cell-associated radioactivity was observed. Scatchard analysis showed that chloroquine increased the...
A single injection of either 5 or 10 mg/kg 8-methoxypsoralen (8-MOP) was given intraperitoneally to male rats at the end of the 14 h light phase (at 2000 h). Two h later (at 2200 h), when the normal nocturnal surge of N-acetyltransferase (NAT) activity and melatonin content in the pineal gland...
Circulating testosterone levels have generally been reported to increase during short term, intense running or bicycle ergometer exercise. Swimming differs from such activity because of the strenuous arm activity and because it is conducted in a liquid medium in the horizontal position with the...
Using partially purified trypsin-solubilized porcine thyroid peroxidase (TPO), an antiserum was developed which inhibited enzyme activity in vitro, and which cross-reacted with partially purified rat TPO. Using rat thyroid minces, this antiserum was utilized to detect radiolabeled TPO in vitro....
This study was designed to confirm the identity of the enzyme involved in the appearance of a nuclear metabolite of 6a—methyl progesterone (6MP) in mouse kidney but not in prostate-seminal vesicle. 6MP and progesterone competed for metabolism by the kidney enzyme. Using progesterone as...
Available androgen binding to soluble proteins from the cytosol of human endometrium was studied using the dextran coated charcoal adsorption method and sucrose density centrifugation analysis. Specific binding of ( 3 H)-5α-dihydrotestosterone (( 3 H)-DHT) was observed with both methods. The...
Effects of 3,3′,5-triiodo-L-thyronine (T 3 ) and dexamethasone (Dex) on the abundance of proteins resolvable by two-dimensional gel electrophoresis were examined in cultured human skin fibroblasts. Confluent cultures were incubated in medium supplemented with thyroidectomized calf serum and...
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