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T-cell receptor excision circles (TREC) may be a useful surrogate marker in HIV-1 infection for evaluating the likelihood of continued clinical stability and/or the response to therapeutics, including vaccines. Analysis of TREC in SHIV and SIV models of HIV-1 infection may provide additional...
Little is known about the cellular mechanisms that control postnatal chondrocyte differentiation. As a first step towards identifying those mechanisms, gene expression shifts were characterized in an in vitro model of chondroinduction. In previous studies, several functional classes of genes...
TIAF1 is a TGF- β 1-induced factor that protects L929 fibroblasts from TNF-mediated apoptosis. In contrast, overexpressed TIAF1 induces growth inhibition and apoptosis of monocytic U937 and various nonfibroblast cells. TIAF1-mediated apoptosis of U937 cells involves upregulation of p53, p21, and...
The transcription factor δ EF1 is a key player in estrogen-signaling cascades in vertebrates. In this pathway, estrogen induces the expression of the gene encoding δ EF1, and then δ EF1 activates transcription of downstream targets. Yet, the molecular mechanisms of transcriptional activation...
We studied expression of protooncogene c- kit receptor in Brown Norway rat Rattus norvegicus testis during different stages of postnatal development. Several regions from within the c- kit gene encompassing different domains were amplified employing reverse transcriptase polymerase chain...
The interactions between C99, presenilin, and nicastrin were investigated by a split-ubiquitin assay. We found that C99 homodimerizes and binds weakly to presenilin and strongly to nicastrin. Domain mapping assays revealed the transmembrane and cytoplasmic carboxy-terminal region of C99 is...
To study the evolution of the solute carrier family 11 (slc11; formerly Nramp) protein, we isolated and characterized two paralogs from the pufferfish Takifugu rubripes ( Fugu ). These teleost genes, designated Fugu slc11a-a and Fugu slc11a-b , comprise open reading frames of 1743 nucleotides...
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