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A method for screening recombinant Î» libraries was devised to select phage containing genomic regions containing provirus insertions of retroviruses that carry the kanamycin and G418 resistance factor neo and the origin of replication derived from pBR322 ( ori pBR ). Such recombinants are...
ABTRACT Using a synthetic deoxyoligonucleotide mixture constructed for a tryptic peptide of the bovine enzyme as a probe, cDNA coding for the full-length subunit of spermidine synthase was isolated from a human decidual cDNA library constructed on phage Î»gt11. After subcloning into the Eco RI...
The expression of the cDNA for alcohol-inducible rabbit liver microsomal cytochrome P-450 form 3a (P450IIE1) in Saccharomyces cerevisiae , with the use of the copper-inducible yeast metallothionein ( CUP1 ) promoter and the ADH1 promoter, is described. Strains 50.L4 and PP1002 were compared for...
Acid maltase or acid Î±-glucosidase (GAA) is a lysosomal enzyme that hydrolyzes glycogen to glucose and is deficient in glycogen storage disease type II. Previously, we isolated a partial cDNA (1.9 kb) for human GAA; we have now used this cDNA to isolate and determine sequence in longer cDNAs...
Glucocorticoids regulate gene expression by causing the glucocorticoid receptor to bind to an enhancer-like DNA element termed the glucocorticoid regulatory element (GRE). The resultant effect on transcription of specific genes causes a cascade of intracellular events that determines the growth...
A cDNA encoding Rev-ErbAÎ±, a member of the thyroid/steroid hormone receptor superfamily, has been isolated from a human fetal skeletal muscle library. This cDNA contains 269 consecutive base pairs identical to a region of a human c-erbAÎ±-2 cDNA, but the respective long open reading frames...
A collection of variant plasmids expressing either Escherichia coli galactokinase or human serum albumin under the control of several E. coli trp promoter derivatives were constructed and studied for both efficiency of expression and regulation by tryptophan. Several variables, including the...
A Î»gt11 human testicular cDNA library was screened with degenerate oligonucleotide probe mixtures based on amino acid sequence data generated from cyanogen bromide fragments and tryptic fragments of purified human Î²-galactosidase. Six positive clones were identified after screening 2 Ã...
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