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Serological assays are routinely used in the laboratory diagnosis of human immunodeficiency virus type‐1 (HIV‐1) infection, but the polymerase chain reaction (PCR) is ultimately the most sensitive and direct method for establishing definitive diagnosis. As an alternative to the conventional...
Calcium flux measurements of different subpopulations of cells by flow cytometry are important in understanding complex interactions in the immune system. This paper discusses the use of the difference of Log signals as a preferred method for obtaining this information simultaneously with other...
Zinc stabilizes membranes and DNA and inhibits respiration in somatic cells. It is present in high concentrations in the male reproductive tract and may stabilize spermatozoa prior to fertilization. Herein, we evaluate N‐(6‐methoxy‐8‐quinolyl)‐p‐toluenesulfonamide (TSQ) for analysis...
Fusion between monocytes and tumor cells has been suggested as a cause for tumor metastasis. The aim of the present study was to establish an in vitro fusion model representing the in vivo situation as close as possible. For this purpose fusion between cells was induced by cytokine containing...
The differentiation of F9 teratocarcinoma cells mimics the formation of a mouse embryonic tissue, the primitive endoderm. In vitro, small aggregates of F9 cells, termed embryoid bodies, differentiate in response to retinoic acid and develop a surface epithelium that is characterized by the...
We evaluate a 1.5 mW HeNe laser (544 nm) for use on an EPICS Elite with a 76 μm Sortsense flow cell. The two applications chosen were immunofluorescence and DNA analysis. We measured the fluorescence threshhold of phycoerytherin calibration beads to be approximately 336 MESF. Cell analysis with...
Apoptotic thymocyte nuclei normally appear on a flow cytometric DNA histogram as a subdiploid peak. We observed that addition of a specific RNase A preparation to the detergent‐based lysing buffer increased the fluorescence of toxicant‐induced apoptotic nuclei to the level of untreated...
The sensitive flow cytometric detection of fluorescent species in liquid sample streams requires efficient collection of light from small (∼1) picoliter (p1) sample volumes. This is often accomplished with high numerical aperture (NA) imaging collection optics used in combination with a spatial...
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