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Fluorescent probes have been utilized to label leukocytes for both in vivo and in vitro studies of cell migration; however, the effects of such probes on migration have not been determined. The aim of this study was to examine the effects of two commonly used fluorescent probes on leukocyte...
Pulse width analysis, in flow cytometry, has been widely used for optimal cell size resolution in cell kinetics analysis. Pulses, generated by scattered light or fluorescence of cells, are electronically analyzed for their height and width. The information generated from these two properties of...
12‐O‐tetradecanoylphorbol‐13‐acetate (TPA) is a potent tumor promoter that causes severe alterations in the biosynthesis of epidermal keratins. This study shows that TPA induces a dyssynchrnous effect on keratin expression in stratified squamous epithelium. The effect of TPA on the...
Human basophils express many clustered differentiation antigens (CD), including CD45; however, none are specific for these cells. In a previous study, we described a two‐color immunofluorescence procedure, employing antibodies to CD45 and IgE for the cytometric evaluation of basophils. In the...
The frequency of micronuclei in binucleated lymphocytes (cytochalasin B assay) may serve as a biological dosimeter after radiation exposure. The automation of the micronucleus assay in binucleated human lymphocytes has been considerably advanced in recent years. In our studies for this purpose...
The sperm chromatin structure assay (SCSA) measures the susceptibility of sperm nuclear DNA to acid‐induced denaturation in situ, and was developed on two Ortho flow cytometers, an FC200 (Becton Dickinson Immunocytometry Systems (BDIS), Westwood, MA) and a Cytofluorograf 30 (BDIS), both having...
A rapid assay was developed for determination of the viability of blastospores of Candida albicans utilizing flow cytometry to detect the accumulation of tetrabromofluorescein in non‐viable yeast cells. Eosin Y was shown to stain non‐viable C. albicans blastospores selectively without...
An investigation of proliferation and activation events in subsets of human CD4+ cells, defined by their expression of CD45RA and CD45R0, is reported. A single‐laser based assay for the study of multiple surface antigens and two‐parameter cell cycle analysis was used for sorting of and...
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