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Feulgen‐DNA and nuclear light green‐protein measurements have been performed in isolated nuclei of normal (nonmalignant) and malignant human endometrial homogenates. The DNA content of the GO/G1 fraction of malignant endometrium showed much overlap with that of normal endometrium, or was...
An estimation of cell kinetic parameters was made by simultaneous flow cytometric measurements of DNA and bromodeoxyuridine (BrdUrd) contents of cells. The procedure described in this paper involves the incorporation of BrdUrd by S phase cells, labeling the BrdUrd with an indirect...
Sources of variation and error were investigated for a simple flow cytometric analysis of DNA content of detergent‐isoiated nuclei stained with ethidium bromide. Using the ploidy classes of mouse liver nuclei, deviations from linearity were assessed for three different instruments. In more...
We have developed a method for quantitatively assessing binding, ingestion, and growth of Chlamydia trachomatis (L2 serovar) in several mammalian cell lines using fluorescence staining and flow cytometry. Cells were incubated with chlamydia at 4°C to monitor binding; ingestion was determined by...
The correlation between flow cytometric ploidy investigation and classic chromosome analysis was studied in 32 human colorectal tumors. Flow cytometry was performed by nuclei isolation and DNA staining with ethidium bromide. Chromosome analysis was done after incubation with colcemid. In 12...
Three pH‐sensitive fluorochromes–4‐methyl‐umbelliferone(4MU),2,3‐dicyano‐hydroquinone (DCH), and 2′,7′‐bis(carboxyethyl)‐5,6‐carboxy fluorescein (BCECF)–were evaluated for their resolution, range, and stability of cellular fluorescence. Flow cytometric techniques for...
Propidium iodide DNA flow cytometry, Feulgen‐DNA, and nuclear light green protein scanning cytometry were performed in columnar epithelial cells of normal, nonmalignant human endometrium and endometrial adenocarcinomas. Columnar cells were identified by immunohistochemical staining for...
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