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Modification of a Coulter EPICS V orthogonal laser‐based flow cytometer/cell sorter allows resolution of X and Y mammalian sperm populations based on DNA content. The modification consists of beveling the sample injection tube situated in the flow chamber, adding a second fluorescence detector...
A method based on detection of drug‐induced cell cycle perturbation by flow cytometric DNA analysis has previously been described in Ehrlich ascites tumors as a way to estimate chemosensitivity. The method is extended to test human small‐cell carcinoma of the lung. Three tumors with different...
With the aim of optimizing in situ hybridization methods, alkaline, acid, and thermal denaturation procedures have been studied for their ability to separate the DNA strands of nuclear DNA and for the DNA losses they induce. Isolated methanol/acetic acid‐fixed mouse liver nuclei have been used...
A data‐smoothing filter has been developed that permits the improvement in accuracy of individual elements of a bivariate flow cytometry (FCM) histogram by making use of data from adjacent elements, a knowledge of the two‐dimensional measurement system point spread function (PSF), and the...
N‐formylated chemotactic peptide stimulation of human neutrophils initiates a number of cellular processes, such as lysosomal enzyme release and superoxide anion production, that are indicative of the events of neutrophil activation during the acute inflammatory response in disease. This study...
We have built a cooling device for cell suspensions in flow cytometry that makes use of the Peltier effect (Barnard RD, Thermo electricity in Metals and Alloys, Taylor and Francis, London; Siemens‐Z 34:383–88, 1963). The prototype described here is used for cooling collection tubes during...
The binding of Hoechst 33258 to rat thymocytes, human lymphocytes, and NHIK 3025 tissue culture cells was studied by measuring the fluorescence and light scattering of the cells as functions of dye concentration using flow cytometry. The results indicated that there were two different modes of...
The phenomenon of leukemic cell maturation requires a measurment of myeloid maturation to understand the process and to exploit it as a means of therapy for leukemia. The HL‐60 leukemic cell line was used as a model of induced leukemic cell maturation in order to develop a method of...
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