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Human genes coding cell surface molecules can be introduced into mouse host cells using a variety of somatic cell genetic techniques. Because these human gene products can be detected using indirect immunofluorescene on viable cells, the genes themsleves can be monitored and manipulated using...
A commercially available flow cytometer (Cytofluorograf) was used for the immunofluorescence (IF) analysis of spores of Bacillus anthracis, Bacillus cereus, and Bacillus subtilis, using fluroescein‐labelled antispore conjugates. The cytometer was modified to allow analysis of known numbers of...
The distribution of single‐cell DNA content was investigated in biopsy specimens from the human cervix of 121 women suspected of having intraepithelial neoplasia. Comparison of the results of the histopathological examination with the ploidy level showed that all normal specimens were diploid....
We report here the application of slit‐scan flow cytometry (SSFCM) in the classification of muntjac, Chinese hamster, and human chromosomes according to centromeric index (CI) and total flurescence. Chromosomes were isolated from mitotic cells, stained with propidium iodide and processed...
The analytic and prognostic value of flow cytometric measurements can generally be improved by preseparation of cells. The pre sent data demonstrate clearly the distinct character of bands obtained in density‐gradient separation. Density‐gradient centrifugation in Percol after extensive Ca2+...
Rapid and reliable identification of various human red cell parasites is important in many chemotherapeutic and immunologic studies. Because manual microscopic counting is tedious and imprecise, we have developed a simple diagnostic procedure for the automated flow cytometric detection of in...
Some 150 tumor specimens from 49 patients with non‐small‐cell carcinoma of the lung (23 epidermoid, 14 adenocarcinoma, 12 large‐cell carcinoma) and three with nonneoplastic lung disease were analysed for cellular DNA content by flow cytometry. Monodispersed cells were stained with ethidium...
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