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Several methods currently in use for measuring mean corpuscular volume include: centrifuged packed cell volume, electronic impedance, and light scattering methods. Although these techniques are widely used and accepted, there are problems inherent to each method which may produce systematic...
A method is described in which the volume, surface area, and diameter of spherical cells can be quickly and easily determined from linear calibration curves using a flow cytometer which measures electronic volume and is equipped with a log amplifier.
Typical mammalian erythrocytes are biconcave disks. Flow cytometric light‐scattering measurements of red cell volume show large, orientation‐dependent variations in signal size. To eliminate errors caused by shape factors, we sphere erythrocytes isovolumetrically on an Auto‐Analyzer...
The reproducibility and accuracy of the Omnicon (Bausch and Lomb Inc., Rochester, NY) automated tumor colony counter for counting tumor colonies growing in double layer soft agar is evaluated and the reproducibility is compared with manual tumor colony counting. Replicate within day...
A nonstoichiometric staining method based on the employment of propidium iodide at a concentration far below saturation is described. The method is particularly suitable for the measurement of staining kinetics and it allowed us to differentiate cell populations with the same DNA content but...
Technical problems have previously prevented the application of fluorescence activated cell sorting to the study of adherent cell populations. We have developed a procedure for attachment of human monocytemacrophages to 14–20 micrometer microspheres. These adherent cells on microspheres...
Current studies of human T lymphocytes and their subsets often use quantitative immunofluorescence analysis with monoclonal antibodies against cell surface antigens. With storage of whole blood or separated mononuclear cells for more than a few hours we have found marked changes in lymphocyte...
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