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The DNA histogram obtained by flow cytometry can be considered as the product of an “ideal” measurement column vector and a measurement distortion matrix. In order to extract the ideal histogram from the real data, the measurement distortion matrix is commonly presumed to be a family of...
Investigations in an experimental model system and in clinical material indicate that squamous bronchial carcinoma is regularly preceded by the occurrence of bronchial cells with DNA values exceeding significantly the normal 4c region. The studies also show that cytomorphological methods do not...
A simple procedure for long‐term storage of cells for flow cytometric DNA analysis was developed and tested. The cells were stored as single cells or fine‐needle aspirates suspended in a citrate buffer with dimethylsulfoxide (DMSO), or as small blocks of tissue from solid tumors. The cells...
Determination of nuclear DNA content by flow cytometry requires comparison with a reference standard. The use of external standards such as lymphocytes or granulocytes is time‐consuming and inaccurate. Chicken red blood cells (CRBC) have a DNA content of 35% of the human diploid value and have...
We have adapted an Ortho ICP‐22 flow cytometer (Ortho Instruments, Westwood MA) for the simultaneous measurement of three independent fluorochromes and cell volume. This has been accomplished by the addition of a third photomultiplier tube and the development of a new electronic cell volume...
Exponentially growing Friend leukemia cells are exposed to 5‐bromodeoxyuridine (BrdUrd) for the time period equivalent to one generation. After fixation and incubation with RNase, DNA in situ is partially denatured by acid and the cells are stained with acridine orange (AO). Staining with AO...
Flow cytometric DNA analysis is an attractive alternative to conventional cytogenetics for diagnosing karyotype changes resulting in abnormal DNA content. We have developed methods for long‐term storage of samples and standards, for staining the cells and for standardization of the measurements...
A video‐computer system has been developed to measure the chronotropy and inotropy of cultured heart cells. The motion of the cell is followed by recording of the changes of light intensity from the edge of a beating cell. With this system the velocityof contraction and relaxation, the time to...
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