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We describe the design and operation of a dual laser fluorescence activated chromosome sorter that can analyze and sort chromosome suspensions stained with two complementary DNA stains. We used two 18‐watt argon‐ion lasers to provide maximal excitation of the DNA‐specific stains Hoechst...
This article focuses on current techniques and possible future developments in chromosome isolation and sorting, and DNA analysis of sorted chromosomes. The strategy of subchromosomal gene mapping by chromosome sorting is outlined and a list of cell lines containing translocated chromosomes is...
In state of the art flow cytometric transducers, the cells are supplied through tubes. Passage through the tube and washing between different samples is time consuming and limits the number of samples that can be processed in a given time. This is a drawback particularly with automatic routine...
Forward angle light scattering of two different wavelengths by cells in a flow cytometer was used to investigate physical differences between lymphocytes of different lineage, functional subclass and developmental stage. Correlation of the ultraviolet (UV: 351 nm and 364 nm) and 488 nm light...
Accumulation of rhodamine B isothyocyanate‐conjugated low density lipoproteins (R‐LDL) in cultured endothelial cells from human umbilical cord was studied with a fluorescence activated cell sorter. R‐LDL uptake was blocked at 0°C, inhibited by addition of excess of nonlabeled low density...
The incorporation of (35S)sulfate and (3H) glucosamine into cetyl pyridinium chloride (CPC) precipitable glycosaminoglycans was determined in B16‐F10 cultured cells sorted with respect to DNA content. Incorporation into surface material was measured indirectly as the difference between the...
Following addition of mitotic inhibitors to asynchronous cultures of mouse lymphoid cell line L1210, the rate of cell exit from G1 phase accelerates during the first 2–3 hr then declines with apparent first order kinetics. The simplest mathematical model that calls for biphasic G1 exit kinetics...
The binding of a fluorescent peptide to human neutrophils is analyzed with a fluorescence activated cell sorter. We examine steady‐state and kinetic features of the ligand‐receptor interaction (in the presence of unbound ligand) and we show that the number of receptors may be estimated...
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