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The mean DNA content per cell and heterogeneity of cell populations with respect to DNA content were measured in samples of peripheral blood mononuclear cells from donors of different ages. Nearly all of the cells in these populations are found in the G1Q phase of the cell cycle. While the mean...
Flow cytometry of testicular tissue from autopsies showed differences in the proportion of substained spermatozoa (1 CA), spermatids (1 CB), diploid 2C nuclei and premeiotic spermatozoa in the 4 C class. The evaluation of the histograms was performed with computer assistance. Healthy accident...
A routine is described that readily allows the rescaling of linear histographic data to a corresponding logarithmic histogram. This procedure significantly improves data display, particularly where a wide range in the measured parameter is encountered. The logarithmic scale displays peaks with...
Normal and transformed fibroblasts can be discriminated by a flow cytometry assay on the basis of their differential reaction with fluorescamine. The cause of altered reactivity of transformed cells with this fluorescent probe has been investigated by a detailed analysis of its reaction with...
Measurements and theoretical calculations of fluorescent emission from four samples of polystyrene microspheres (diameter 0.92, 1.63, 1.90 and 4.18 &muml;m) containing the same fluorescent dye show a general dependence upon particle size, emission angle, and polarization conditions. However, for...
To determine the change in nuclear protein content as a function of cell cycle position, isolated heLa nuclei were stained for protein with fluorescein isothiocyanate (FITC) and for DNA with propidium iodide (PI) and analyzed by flow cytometry (FCM). The resulting FITC versus PI histogram...
C3H/He‐mg and AKR mice were injected intraperitoneally with lymphocytes prepared from the spleen of (C3H/He‐mg X AKR)F1 hybrid mice bearing Gross‐virus leukemia. Lymphocyte suspensions were prepared from the spleen, mesenteric lymph node and thymus of mice at different days after the...
Detailed, simplified techniques are described for simultaneous staining and analysis of DNA and protein in a number of mammalian cell types. Cell staining involves the addition of appropriate dye mixtures to unfixed or ethanol‐fixed cells and subsequent analysis of cell populations in the...
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