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Highlights ► Advances in split-proteins using PET, FRET and BRET strategies. ► Interrogating small molecule inhibitors. ► Detection of native proteins, DNA, RNA, Fe–S clusters, PAR and small molecules. ► Chemical inducers of dimerization strategies to control split-protein activity. ►...
Highlights ► Diversity generating methods differ depending on the material of imaging probe. ► Unbiased screening of fluorophore and binding enzyme revealed novel imaging tags. ► High-throughput screening of diversity-oriented library disclose novel imaging probes. ► Careful design of...
Highlights ► Chromatin substructures fall into a resolution gap between EM and optical microscopy. ► Super-resolution fluorescence microscopy is well suited to access these substructures. ► We review new insights into chromatin structure gained by super-resolution imaging. ► We discuss...
Highlights ► Recent advances in visualizing protein associations under native conditions. ► Advances focus on reducing background, optimizing signal, improving kinetics. ► Others focus on decreasing cytotoxicity and minimizing functional perturbation.
Highlights ► We review advances in TCCD, force spectroscopy, and smFRET single-molecule techniques. ► Obtaining structural models of telomerase assembly and function are a persistent challenge. ► We review studies on telomerase assembly, RNA folding, structure, and activity. ►...
Highlights ► Pre-mRNA splicing is carried out by a MegaDalton machine called the spliceosome. ► Single molecule fluorescence combined with chemical biology techniques has recently enabled real time visualization of splicing and spliceosome assembly. ► Single molecule FRET measurements...
Highlights ► Explanation of the need for tools to image protease activity. ► Examples of recently developed substrate-based imaging probes for cysteine proteases. ► Examples of recently developed activity-based imaging probes for cysteine proteases. ► Discussion of the pros and cons of...
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