Within the last year
Within the past 3 years
1 - 10 of 19 articles
Highlights ► Advances in split-proteins using PET, FRET and BRET strategies. ► Interrogating small molecule inhibitors. ► Detection of native proteins, DNA, RNA, Fe–S clusters, PAR and small molecules. ► Chemical inducers of dimerization strategies to control split-protein activity. ►...
Highlights ► Diversity generating methods differ depending on the material of imaging probe. ► Unbiased screening of fluorophore and binding enzyme revealed novel imaging tags. ► High-throughput screening of diversity-oriented library disclose novel imaging probes. ► Careful design of...
Highlights ► Chromatin substructures fall into a resolution gap between EM and optical microscopy. ► Super-resolution fluorescence microscopy is well suited to access these substructures. ► We review new insights into chromatin structure gained by super-resolution imaging. ► We discuss...
Highlights ► We review advances in TCCD, force spectroscopy, and smFRET single-molecule techniques. ► Obtaining structural models of telomerase assembly and function are a persistent challenge. ► We review studies on telomerase assembly, RNA folding, structure, and activity. ►...
Highlights ► Recent advances in visualizing protein associations under native conditions. ► Advances focus on reducing background, optimizing signal, improving kinetics. ► Others focus on decreasing cytotoxicity and minimizing functional perturbation.
Highlights ► Pre-mRNA splicing is carried out by a MegaDalton machine called the spliceosome. ► Single molecule fluorescence combined with chemical biology techniques has recently enabled real time visualization of splicing and spliceosome assembly. ► Single molecule FRET measurements...
Highlights ► Explanation of the need for tools to image protease activity. ► Examples of recently developed substrate-based imaging probes for cysteine proteases. ► Examples of recently developed activity-based imaging probes for cysteine proteases. ► Discussion of the pros and cons of...
Read and print from thousands of top scholarly journals.
Sign up with Facebook
Sign up with Google
Already have an account? Log in
Save this article to read later. You can see your Read Later on your DeepDyve homepage.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Sign Up Log In
To subscribe to email alerts, please log in first, or sign up for a DeepDyve account if you don’t already have one.
To get new article updates from a journal on your personalized homepage, please log in first, or sign up for a DeepDyve account if you don’t already have one.