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Although the use of microbial biocatalysts for chemical reaction pre-dates by a considerable margin the application of isolated enzymes for this purpose, considerable progress continues to be made in the use of whole-cell catalysts. The application of whole-cell biocatalysts in...
Protein engineering is the application of knowledge to design and alter protein function and structure. Although powerful methods, from specific to random, have been developed for the redesign of protein architecture, their successful application is dependent on the information known about the...
The contributions of cell surface oligosaccharides to critical biological processes such as leukocyte—endothelial cell adhesion, bacterial and viral infection, and immunological recognition of tumor cells and foreign tissue are now understood in significant molecular detail. These discoveries...
The major endoproteolytic processing enzymes of the secretory pathway are the subtilisin-like proprotein convertases (SPCs). Furin (SPC1) has emerged as one of the major processing enzymes of the constitutive secretory pathway and its localization in the trans -Golgi network and mechanism of...
Metabolic engineering is a rapidly evolving field. The term typically refers to the genetic modification of cellular biochemistry to introduce new properties or modify existing ones. Recent progress in genetics, molecular biology, microbiology and chemistry are driving advances in this field....
DNA binding and transcriptional activation by basic region leucine zipper (bZIP) proteins can be enhanced by accessory factors. In the past year, three accessory factor—bZIP—DNA complexes have been investigated extensively. Experiments have probed the effect of accessory factors on bZIP—DNA...
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