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Protein–DNA binding assays have been used in a variety of fields from fundamental studies regarding the binding process itself, to serving as probes for the detection, quantification and separation of target analytes. These assays have been used for the study of protein–DNA complex...
Mitochondria are both morphologically and functionally diverse, and this variety is thought to have important biological ramifications. The development of methods to probe the properties of individual mitochondria is therefore of utmost importance. Recent advances have been made using in situ...
Developing the ability to quantify changes in protein abundance between cells subjected to a variety of physiological and environmental conditions is an extremely active area of proteome research. Although advances in chromatography, mass spectrometry instrumentation, and bioinformatics have...
The high-throughput identification and accurate quantification of proteins are essential components of proteomic strategies for studying cellular functions and processes. Techniques that are largely based on stable isotope protein or peptide labeling and automated tandem mass spectrometry are...
The Human Genome has been sequenced in large part owing to the invention of capillary electrophoresis. Although this technology has matured enough to allow such amazing achievements, the physical mechanisms at play during separation have yet to be completely understood and optimized. Recently,...
Recent research to improve matrices for DNA separation has resulted in the development of advanced polymers for use in capillary electrophoresis and, more generally, for electrophoresis in microchannels. To date, the most commonly used matrix is linear polyacrylamide (LPA). Unfortunately, the...
Multiplexed immunoassays on antibody-based protein microarrays are an attractive solution for analyzing biological responses in normal and diseased states. Recently, the feasibility and utility of these assays has been established as concerns about specificity and sensitivity are being overcome...
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