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Abstract An automated fluorometric microtechnic, suitable for screening purposes, has been developed for the quantitative determination of glucose-6-phosphate dehydrogenase (G6PD) and 6-phosphogluconate dehydrogenase (6PGD) levels in red blood cells. The method is rapid, simple, and precise. It...
Abstract Data are presented from the study of a new systematized, kinetic method for the determination of alkaline phosphatase* which was run concurrently in three geographically separated laboratories. The data include estimates of within-day and day-to-day precision, and...
Abstract A new spectrophotometric method for the determination of serum glutamic oxalacetic transaminase§ (SGOT) is described which uses standardized technics and prepackaged reagents. A study was made of this new method in three laboratories and was compared with the routine methods, using...
Abstract A new, atomic absorption spectrophotometric method for serum iron and iron-binding capacity is described, which utilizes 20% (w/v) TCA plus heating at 90° for 15 min. This procedure liberates the ferric iron; precipitates the protein, facilitating removal by centrifugation; and avoids...
Abstract The ninhydrin-colorimetric procedure for the determination of urinary α amino-nitrogen has been simplified by the use of the Conway microdiffusion unit to remove ammonia from the sample. In this way, urinary ammonia is measured, and at the same time an ammonia-free urine sample is ready...
Abstract A simple automated procedure for total serum cholesterol, employing a stable Liebermann-Burchard reagent, was tested. The method has good precision, and results agree well with extraction methods for total cholesterol on normal serums. Serum samples with elevated bilirubin levels gave...
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