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Abstract An automated system for the determination of the L-α form of the majority of amino acids is presented. The method is based upon oxidative deamination of the amino acid coupled with oxidation of o-dianisidine by hydrogen peroxide. This procedure can be used comparatively for the...
Abstract A kinetic method is described for the determination of γ-glutamyl transpeptidase activity in serum. Optimal conditions for the reaction were ascertained, normal values in milliunits per milliliter at 25° established, and the reliability of the method examined....
Abstract A detailed description of a method for the determination of serum arginase activity is presented. Arginase activity is demonstrated by the decrease in arginine concentration in a reaction mixture, as determined by a modification of the method of Sakaguchi. The reaction is linear until...
Abstract An automated fluorometric procedure, using AutoAnalyzer modules and based on the alcohol dehydrogenase reaction, has been developed for the determination of ethanol in fingertip capillary blood samples. Whole blood is measured directly from the finger prick into 0.02-ml. capacity...
Abstract Using the Kober-lttrich fluorescence procedure, automated fluorometry was applied to the assay of individual estrogens and their 3-methyl ether derivatives in the purified urinary extracts of nonpregnant women. The values obtained by using this new automated scheme were compared with...
Abstract An efficient summation technic for the quantitation of total lipid from the results of the cholesterol, phospholipid, and triglycerides determinations is compared to the phenol turbidity and gravimetric method. Also considered is the total lipid value calculated from the cholesterol and...
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