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Abstract Two minor hemoglobins present in normal human erythrocytes have been separated by paper electrophoresis. The concentration of the component which was more positively charged than the bulk hemoglobin ranged between 1.5 and 6 per cent of the total hemoglobin. The concentration of the...
Abstract An apparatus is described for paper electrophoresis according to the moist-chamber principle. A simple method for quantitative evaluation of the different electrophoretic fractions is presented together with the limits for the normal variation of the different serum fractions. The...
Abstract A simple method for the assay of urinary peptides has been presented. This method involves the reaction of a biuret reagent on a tungsticacid filtrate of Permutit-treated urine. Some possible interfering substances have been investigated and the precision of the method has been...
Abstract A method for determining serum lipase activity requiring only 4 hours incubation at 37° is given. Olive oil in a phosphate buffer is the substrate. The values found in 57 healthy controls ranged between 0.06 and 0.87 units, with a mean of 0.31 ± 0.17 (S.D.). Some results are given for...
Abstract Yeast adenylic acid was investigated as a substrate for serum acid phosphatase determination. It was compared as a substrate primarily with β-glycerophosphate and found to be very similar in selectivity and sensitivity. There appears to be no significant advantage in the use of yeast...
Abstract A method for the determination of serum sodium and potassium involving only a single serum dilution is described. Samples of 0.1 ml. of serum suffice for both determinations, but larger samples may also be used. By careful control of the lithium concentration and the dilution factor the...
Abstract 1. The amount of cystine was determined in a number of serum protein fractions separated by the procedure of E. J. Cohn. In albumin, the average value was 6.40 per cent for cystine; for γ-globulin the average value was 1.94 per cent; the other fractions tested were mixtures and varied...
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