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A high-performance liquid chromatographic method with evaporative light-scattering detection (ELSD) has been developed for analysis of spectinomycin and related impurities. Separation of spectinomycin from structurally related impurities was achieved on a C 18 column. The optimized mobile phase...
The occurrence of antibiotics in the aquatic environment is an important emerging issue due to potential adverse effect of these compounds on ecosystem and human health. For a correct environmental risk assessment there is a need for appropriate analytical methods for monitoring antibiotic...
We have developed and validated a simple HPLC method for analysis of leflunomide in tablets. Method conditions were determined by assay of a photodegraded sample of leflunomide. Optimum chromatographic performance was obtained with a C 18 column and acetonitrile-water as mobile phase. Comparison...
For the first time a high-performance liquid chromatographic (HPLC) method, with liquid-liquid extraction and ultraviolet (UV) absorbance detection, has been developed for quantification of isofraxidin in rat plasma. The analysis was performed on a Diamonsil C 18 column (200 mm × 4.6 mm i.d., 5...
A specific, sensitive and precise liquid chromatographic assay method was established using LC-MS for the determination of acyclovir (ACV) in aqueous humor (AH), which was directly injected onto an Inertsil ODS-3 C 18 column without any pretreatment. The Agilent 1100 series LC–MS system was...
In the present study a multi-residue analytical method was developed for monitoring some polar pesticides such as acephate, methamidophos, carbofuran, isoproturon, dimethoate in water with SPE (solid-phase extraction) and LC–MS–MS. Acetochlor was taken as surrogate, and alachlor as internal...
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