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In this paper we report on high resolution capillary electrophoresis separation of structural isomers of oligosaccharides of biotechnology interest. Analysis of carbohydrate constituents of various plant and food sources of bioindustrial significance requires high-resolution characterization...
The effectiveness of diethylentriamine (DIEN) phosphate buffer at separating basic proteins in bare fused-silica capillaries at various pH values was examined. Such a buffer consists of an aqueous solution of DIEN titrated to the desired pH value with phosphoric acid. The study was conducted by...
This paper is written in remembrance of the work of Csaba Horváth on Reversed Phase Chromatography, (RPC) and the fundamental theory of the mechanism of retention on non-polar stationary phases, the ``Solvophobic Theory''. The paper discusses some steps in the development of this important...
Monolithic capillary columns were prepared by thermal initiated copolymerization of glycidyl methacrylate (GMA) and divinylbenzene (DVB) inside silanized 200 µm i.d. fused silica capillaries. Polymerization mixtures containing different amounts of porogen (1-decanol and tetrahydrofuran (THF))...
The first results of the combination of BioArena and OPLC with flowing eluent wall technique (FEW) show an especially attractive solution of the detection of antimicrobial activity and compound isolation based on peak collection. Because of the different separation mechanisms applied for on-line...
The advantages and variations of thin-layer chromatography are described in detail. The displacement mode of development is generated in a relatively short distance, and the fully developed displacement train concentrates sample components even from an elongated spot. Two-dimensional TLC using...
The nature of surface silanols is reviewed and their influence on retention of basic solutes in reversed phase chromatography is demonstrated and evaluated. The influence of the type of organic modifier and/or pH of buffer on retention of basic analytes with silica based RP columns is evaluated,...
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