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The most recent interest in liquid chromatography (LC) is the trend of column miniaturization, i.e., in reducing the diameter of the columns. Capillary columns used in LC can be classified into three types, based on the flow resistance factor ϕ defined by Knox as where d is a characteristic...
HPLC is the most convenient method for the assay of the azithromycin (10-dihydro-10-deoxo-11-methyl-11-azaerythromycin A), a semisynthetic macrolide antibiotic and its reaction intermediates. Data concerning the effects of pH, temperature and column type are presented. The analytical procedures...
The thermodynamic description of the capacity factor (k′), the most widely used retention parameter, is presented. The connection of the thermodynamic adsorption equilibrium constant (K) and the retention parameters are shown. Methods for dead volume determination are described. A general...
Retention prediction of phenythiohydantoin amino acid derivatives in isocratic reversed-phase liquid chromatography was investigated. The predicted retention data of all derivatives were evaluated by comparing them with actually measured retention data. Excellent agreements between these data...
The allyl bonded phase is used as an intermediate in the synthesis of a weak cation exchange material. The double bond is first converted to an aldehyde by an ozonation reaction and then subsequent oxidation to the carboxylate group is accomplished with dichromate. Diffuse reflectance Fourier...
Two close-boiling materials, diethyl ether (DEE) and dichloromethane (DCM), were separated to investigate the effects of the pure components and the mixture on elution in preparative gas-liquid chromatography. Nitrogen was used as the carrier gas, and the chromatographic column (1 cm I.D. and...
The analysis of ethinyloestradiol in tablets has been carried out by reversed phase high performance liquid chromatography (HPLC) using electrochemical detection. The drug was eluted with aqueous acetonitrile containing sodium acetate as background electrolyte. The mobile phase was used as...
Partition chromatographic behaviour of the rare earth elements on C 18 bonded silica reversed-phase material has been investigated by thin-layer chromatography in methanol — lactate media. The rare earth lactato complexes are distributed and fractionated on bonded silica layers without...
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