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To produce standards for GPC calibration, oligomers of polyacenaphthylene were fractionated by the method of repeated sample injection in an analytical GPC unit (μ-styragel-columns, eluent THF). It is possible to separate about 2 g polymers in 24 hours into fractions with good uniformity.
The on-column oxidation of hydrocarbons commonly used as stationary phases in column characterization is investigated. In each case significant shifts of retention are observed, which may be negative or positive depending upon the polarity of the test solutes and the adsorptivity of the support...
An attempt was made to apply a low-activity chromatographic layer (Whatman 2) and a non-polar mobile phase (decalin) in studying the self-association of higher fatty alcohols. The obtained results showed a satisfactory, semi-quantitative agreement with IR spectroscopic data and theoretical...
Reversed-phase systems using octyl modified silica as such and as a support for dynamically coated ion-exchangers, were investigated for their ability to separate pentapeptides. Normal reversed-phase adsorption with C-8 bonded silica in combination with citrate bufferpropanol-1 mixtures were...
The analyses of the plasma concentrations of two drugs, one a water soluble weak base and the other a lipophilic ester with acidic metabolites, are used to illustrate the advantages of reversed-phase chromatography over other techniques in drug metabolism studies. The problems associated with...
Thin-layer chromatography has been performed on plates spread with polystyrene-imminodiacetate-exchanger (Chelex 100) bound to the ammonia complexes of different metal ions such as nickel(II), zinc(II), copper(II) and cobalt(III). A ligand-exchange effect was evidenced in the separation of some...
A rapid, sensitive and direct method is described for the detemination of ethanol in “Alcohol-Free” beverages and fruit juices. A known amount of internal standard is added to the sample and a 0.1 mm 3 aliquot is injected directly into a gas chromatograph. Ethanol concentrations as low as...
A method is reported for the separation of 16 closely related 2-(1′, 2′-diphenylvinyl)-3-phenylbenzofuran and 2-(1′, 2′-diphenylvinyl)-1-phenylnaphtho(2,1-b) furan derivatives on thin-layers of silica gel-G.
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