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Abstract Statistical methods such as regression analysis are often used to estimate cell kinetic parameters, such as cell birth rate or tumour growth rate. the standard errors which the statistical procedures provide may be considerable underestimates of the true uncertainty surrounding the...
Abstract Tritium-labelled uridine ((3H)UdR) perturbs progression of L1210 cells through the mitotic cycle. the main effect manifests as a slowdown or arrest of a portion of cells in G2 and is already observed 2 hr after addition of 0.5–5.0 μCi/ml of (3H)UdR into cultures. At 2.5–5.0 μCi/ml...
Abstract Various types of mathematical models, such as partial differential equations, ordinary differential equations and difference equations, are available in the literature to describe the kinetics of cell proliferation, and different studies of cell kinetic phenomena have been conducted...
Abstract Erythropoiesis in vitro was studied with practically pure erythroid progenitor cells: CFU-E (colony-forming-units-erythroid). the isolation of CFU-e from spleens of thiamphenicol pretreated anaemic mice with the combined methods of centrifugal elutriation and Percoll density gradient...
Abstract Lens epithelium, when attached to its natural substratum, the lens capsule, can be maintained in culture for more than 2 weeks in a simple HEPES- and EDTA-buffered salt solution (HBS). In HBS, the epithelium shows the same characteristic phenomena of locomotion, initial retraction and...
Abstract A human leukaemic cell line (REH) growing in suspension was incubated with cis-platinum, hydroxyurea and mitomycin C at various concentrations causing complete cell-cycle arrest. At different times the cell suspensions were harvested, diluted 1:1 with a buffer solution, stained without...
Abstract The extraordinary sensitivity of early erythroid progenitor cells (BFU-e) of normal human bone marrow to tritiated thymidine ((3H)TdR) was studied. While exposure of bone-marrow cells to (3H)TdR for 1 hr resulted in the death of only 40% of the granulocyte-macrophage progenitor cells...
Abstract The aim of the present investigation was to study the effect of a high bolus injection (1 × 2.1 mg) of vincristine (VCR) during the phase of tumour growth retardation at the 14th day after transplantation and to compare the findings with the results of single (1 × 0.35 mg) and repeated...
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