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We studied the role of VEGF signal pathway in autocrine regulation of tumor cell growth and survival under conditions of hypoxia. Hypoxia-resistant CaOv/H substrain with high level of VEGF-A secretion was obtained by long-term culturing of CaOv ovarian adenocarcinoma cells with CoCl 2 (hypoxia...
A method for double immunoperoxidase staining of the blood-brain barrier cell elements was developed using 2mB6 monoclonal antibodies specifically visualizing brain capillary antigen and antibodies to glial fibrillary acidic protein (GFAP). The method is based on consecutive visualization of...
Peculiarities of active avoidance conditioning were studied in NISAG rats (hereditary stress-induced arterial hypertension) and spontaneously hypertensive SHR rats. Conditioning was successful in 90% normotensive Wistar rats and in only 9.1% NISAG rats. Hypertensive SHR rats were intermediate...
Comparative analysis of the expression of some surface markers of human bone marrow mesenchymal stem cells, umbilical fibroblast-like cells, and skin fibroblasts was carried out by the flow cytofluorometry method. Mesenchymal stem cells and umbilical fibroblast-like cells were similar by the...
The relationships between embryonic stem and cambial cells in the ontogeny were evaluated on the basis of our data on organ embryogenesis and in vivo implantation of epithelial tissues and published data. We demonstrated the role of recipient tissues in the implantation process. Aseptic...
Slight cooling had no effect on heat emission and heat production, but modulated the immune response to antigen in animals. Changes in the immune response upon rapid slight cooling of the skin (by 1.5°C) depended on the phase of immunogenesis corresponding to cold exposure. When cooling was...
Oral treatment with chitosan with a molecular weight ∼10 5 , but not its oligomer, reduced plasma content of free-radical oxidation products in normal rats and animals treated for the bone marrow form of radiation sickness and stimulated the recovery processes in involved bone marrow and...
The charge properties of cryoglobulins were examined during the first 21 days of ischemic stroke (atherothrombotic and cardioembolic). Routine drug therapy produced no effect on the charge of cryoglobulins. Plasmapheresis significantly modified the electrokinetic parameters of cryoglobulins...
We present a technology of culturing of human mesenchymal stem cells under conditions excluding the presence of animal sera or additional growth factors, but preserving high proliferative potential and the capacity of these cells to multilineage differentiation. Human umbilical serum was used as...
Serum concentration of L-carnitine, the mean thickness of the skeletal muscle fiber, and exercise performance in the forced swimming test decreased in rats receiving a carnitine-deficient diet. Treatment with L-carnitine compensated for carnitine deficiency, while racemate and D-stereoisomer did...
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