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The cDNA encoding for lignin peroxidase of Phanerochaete chrysosporium was expressed in the Pichia methanolica under the control of the alcohol oxidase (AUG1) promoter which was followed by either the lignin peroxidase leader peptide of Phanerochaete chrysosporium or the Saccharomyces cerevisiae...
Peptides containing Arg-Gly-Asp (RGD) have been used to decrease thrombosis by competitive inhibition of the integrin glycoprotein, αIIb/β3a, in platelets. However, they have a short half-life in vivo . A naked plasmid, pCMV-RGD, was transferred into the skeletal muscle of mice and RGD gene...
Compared to glutathione S -transferase (GST), tagging with hexahistidine residues (His) has several merits: low levels of toxicity and immunogenicity, a smaller size and no electric charge. We have constructed a novel expression vector, designated as pHisJM (EMBL/GenBank/DDJB accession no....
The thermophilic bacterium, Moorella sp. HUC22-1, newly isolated from a mud sample, produced ethanol from H 2 and CO 2 during growth at 55 °C. In batch cultures in serum bottles, 1.5 mm ethanol was produced from 270 mm H 2 and 130 mm CO 2 after 156 h, whereas less than 1 mm ethanol was produced...
Rice and wheat brans, without additional nutrients and hydrolyzed by α-amylase and amyloglucosidase, were fermented to dl-lactic acid using a newly isolated strain of Lactobacillus sp. RKY2. In batch fermentations at 36 °C and pH 6, the amount of lactic acid in fermentation broth reached 129 g...
The optimum growth stage for enhancing ajmalicine production in Catharanthus roseus cultures with methyl jasmonate (MJ) was after 6 d growth. MJ added at 10 or 100 μm on day 6 gave a maximum ajmalicine production of 10.2 mg l −1 , a 300% increase over that of non-elicited cultures.
Genetic manipulation of embryonic stem (ES) cells is performed by non-viral as well as viral transfection methods. We tested the recently developed nucleofection method delivering plasmid DNA directly into the nucleus for the introduction of a plasmid encoding enhanced green fluorescent protein...
Trehalose added to storage buffers for chemical-induced transformations at 200 mm increased the survival of Escherichia coli competent cells 1.3∼4.8-fold over 180 d cryostorage compared with dimethyl sulfoxide or glycerol.
Acetic acid is by-product from fermentation processes for producing succinic acid using Mannheimia succiniciproducens . To obtain pure succinic acid from the final fermentation broth, acetic acid was selectively removed based on the different extractability of succinic acid and acetic acid with...
A polyhydroxyalkanote (PHA) depolymerase gene ( pha Z) was cloned by PCR from Pseudomonas putida and over-expressed in Escherichia coli as inclusion bodies. Nucleotide sequence analysis predicted an 852 bp open reading frame encoding a protein of 283 amino acids with a predicted molecular weight...
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