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“Seeing is believing” – this issue of Biotechnology Journal features a collection of articles on the latest in imaging technologies.
Modification of proteins with small molecules is a widely used and powerful tool in biological research. Enzymatic approaches are particularly promising because substrate specificity allows for site‐specific modification. Sortase A, a transpeptidase from Staphylococcus aureus, cleaves between...
Precise histopathological localization of E‐cadherin and p63 is of immense importance in understanding the integrity of oral mucosal stratified epithelium in normal and diseased conditions. Necessarily immunohistochemical imaging should have minimum bleaching impact on the dyes and ability to...
PEGylation is the most successful approach, to date, to prolong the in vivo survival of recombinant proteins. The conjugation of the polymer to glycoproteins results in challenging analysis, and furthermore, requires a wide variety of analytical tools for the determination of the extent of...
The book edited by Stefan Stamm, Chris Smith and Reinhard Lührmann, “Alternative pre‐mRNA splicing, theory and Protocols”, has the ambition to provide a very large compendium of both theory and applications in pre‐mRNA splicing analysis for all biology and biochemistry investigators and...
Glutathione is a valuable tripeptide that is widely used in the pharmaceutical, food, and cosmetic industries. Glutathione is industrially produced by fermentation using Saccharomyces cerevisiae. Before the glutathione fermentation process with S. cerevisiae, a glucose extraction process from...
Rayleigh‐Bénard convective PCR is a simple and effective design for amplification of DNA. Convective PCR is, however, extremely sensitive to environmental temperature fluctuations, especially when using small‐ diameter test tubes. Therefore, this method is inherently unstable with limited...
Focus: Imaging technology. This issue of Biotechnology Journal features a collection of articles on the latest in imaging technologies, such as CARS microscopy to show lipid synthesis pathways, ApoTome imaging in immunohistochemistry, and functional imaging of microbial physiology. Image: ©...
Type I and II secretory pathways are used for the translocation of recombinant proteins from the cytoplasm of Escherichia coli. The purpose of this study was to evaluate four signal peptides (HlyA, TorA, GeneIII, and PelB), representing the most common secretion pathways in E. coli, for their...
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