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Tissue engineering and regenerative medicine aim to produce tissue substitutes to restore lost functions of tissues and organs. This includes cell therapies, induction of tissue/organ regeneration by biologically active molecules, or transplantation of in vitro grown tissues. This review article...
In the pharmaceutical industry, it is state of the art to produce recombinant proteins and antibodies with animal‐cell cultures using bioreactors with volumes of up to 20 m3. Recent guidelines and position papers for the industry by the US FDA and the European Medicines Agency stress the...
The objective of the present study was to investigate the effect of hydrodynamic stress heterogeneity on metabolism and productivity of an industrial mammalian cell line. For this purpose, a novel Lobed Taylor‐Couette (LTC) mixing unit combining a narrow distribution of hydrodynamic stresses...
Although the relevance of three‐dimensional (3‐D) culture has been recognized for years and exploited at an academic level, its translation to industrial applications has been slow. The development of reliable high‐throughput technologies is clearly a prerequisite for the industrial...
Over the last few decades, high‐throughput (HT) bioscreening, a technique that allows rapid screening of biochemical compound libraries against biological targets, has been widely used in drug discovery, stem cell research, development of new biomaterials, and genomics research. To achieve...
Cover illustration Scaffold‐free cell‐based systems. This issue of BTJ, edited by Umut Gurkan (Harvard Medical School, MA, USA) and Feng Xu (Xi' an Jiaotong University, China) highlights several applications of scaffold‐free systems such as cell therapies for tissue regeneration, in vitro...
Bacillus megaterium was used as an alternative high potential microbial production system for the production of antibody fragment D1.3 scFv. The aim of the study was to follow a holistic optimization approach from medium screening in small scale microtiter platforms, gaining deeper process...
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