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We describe the cloning and characterization of a mutated thermostable DNA polymerase from Thermus aquaticus (Taq) that exhibits an increased reverse transcriptase activity and is therefore designated for one‐step PCR pathogen detection using established real‐time detection methods. We...
In this study, nanoporous gold supercapacitors were produced by electrochemical dealloying of gold‐silver alloy. Scanning electron microscopy and energy dispersive X‐ray spectroscopy confirmed completion of the dealloying process and generation of a porous gold material with ∼10 nm diameter...
Hepatocyte function on 3‐D microfabricated polymer scaffolds realised with the pressure‐activated microsyringe was tested under static and dynamic conditions. The dynamic cell culture was obtained using the multicompartment modular bioreactor system. Hepatocyte cell density, glucose...
Barley, a green ligand factoryErlendsson et al., Biotechnol. J. 2009, 5, 163–171Growth factors are commonly used as cell culture supplements and might gain even more importance in stem cell research, tissue engineering and regenerative medicine or as human therapeutics. Recombinant growth...
BTJ best cover contest Biotech paper watch: Triple knockout cell lines in one go Turning carbon dioxide into liquid fuel Dual malaria/cholera vaccines from chloroplast Automated single cell clone picking Disulfide trapping of yeast surface proteins pH changes upon freezing of buffered solutions...
The cost‐effective production of biofuels from renewable materials will begin to address energy security and climate change concerns. Ethanol, naturally produced by microorganisms, is currently the major biofuel in the transportation sector. However, its low energy content and incompatibility...
Extraction of high‐quality RNA from Arabidopsis seeds has been a challenge. Here we report a two‐step TRIzol‐based procedure for RNA extraction from Arabidopsis siliques and dry seeds. This procedure employs a modified, high pH (pH 9.5) extraction buffer. High pH plus the addition of either...
Substantial equivalence has become established as a foundation concept in the safety evaluation of transgenic crops. In the case of a food and feed crop, no single variety is considered the standard for safety or nutrition, so the substantial equivalence of transgenic crops is investigated...
Where 2A oligopeptide sequences occur within ORFs, the formation of the glycyl–prolyl peptide bond at the C‐terminus of (each) 2A does not occur. This property can be used to concatenate sequences encoding several proteins into a single ORF: each component of such an artificial polyprotein is...
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