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News: Plastics from E. coli – Looking again (carefully) at thalidomide – Don't kiss the chimps – Imaging microbial interactions – Probiotics prevent intestinal diseases – Drug resistance in cancer stem cell populations – Triple tag technique turns up tight and loose complexes – It...
Since the first PEGylated product was approved by the Food and Drug Administration in 1990, PEGylation has been widely used as a post‐production modification methodology for improving biomedical efficacy and physicochemical properties of therapeutic proteins. Applicability and safety of this...
The substantial progress in embryonic stem cell (ESC) research could lead to new possibilities in the treatment of various diseases. Currently, applications of ESC for cell therapy are impeded by the presence of potentially teratoma‐forming undifferentiated ESC. Thus, a selective and...
Recent outbreaks of influenza A highlight the importance of rapid and sufficient supply for pandemic and inter‐pandemic vaccines. Classical manufacturing methods for influenza vaccines fail to satisfy this demand. Alternatively, cell culture‐based production systems and virus‐like particle...
We report a method for the integration of expression cassettes into the Escherichia coli chromosome using rare and dispensable sugar degradation gene loci as sites for integration. Clones carrying successfully recombined DNA fragments in the chromosome are easily screened using a solid...
Assay validation was performed using four consecutive experiments with the related statistical evaluation. A cell‐based assay on microtitre plates measured repeatedly within 1 day and on consecutive days was chosen as the model. The following problems were addressed: (i) choosing an appropriate...
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