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Bactericidal effects of blood platelets have been known for more than 120 years, but the underlying mechanisms are largely obscure. Keeping in mind structural and functional analogies of platelets to neutrophils, three different mechanisms are thinkable: Engulfment of pathogens, release of...
Cover illustration: Imaging host‐pathogen interactions. This special issue of Biotechnology Journal contains selected contributions from participants of a symposium that took place in September 2008 in Heidelberg, Germany. Guest editors are Spencer Shorte (Institut Pasteur, Paris) and Freddy...
Super‐resolution imaging allows the imaging of fluorescently labeled probes at a resolution of just tens of nanometers, surpassing classic light microscopy by at least one order of magnitude. Recent advances such as the development of photo‐switchable fluorophores, high‐sensitivity...
We recently developed an approach combining fluorescence in situ hybridization (FISH) and flow cytometry for detecting low levels of Salmonella spp. (∼103 cells/mL sprout wash) against high levels of naturally occurring sprout flora (∼107–108 CFU/g sprouts). Although this “FISH and...
Over millions of years pathogens have coevolved with their respective hosts utilizing host cell functions for survival and replication. Despite remarkable progress in developing antibiotics and vaccination strategies in the last century, infectious diseases still remain a severe threat to human...
Studying the events that occur when a pathogen comes into contact with its host is the basis of the field of infection biology. Over the years, work in this area has revealed many facets of the infection process, including attachment, invasion and colonization by the pathogen, and of the host...
Biological samples are three dimensional and, therefore, optical sectioning is mandatory for microscopic images to precisely show the localization or function of structures within biological samples. Today, researchers can choose from a variety of methods to obtain optical sections. This article...
Multiphoton imaging of host‐pathogen interactionsMelican and Richter‐Dahlfors, Biotechnol. J. 2009, 4, 804–811Many biochemical interactions between pathogens and their host cells have been studied using in vitro assays or cultivated cells. State‐of‐the‐art imaging techniques aim at...
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