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The dead Kluyveromyces marxianus biomass, a fermentation industry waste, was used to explore its sorption potential for lead, mercury, arsenic, cobalt, and cadmium as a function of pH, biosorbent dosage, contact time, agitation speed, and initial metal concentration. The equilibrium data fitted...
Special focus: Synthetic biologyWhat is synthetic biology?SynBERC – The Synthetic Biology Engineering Research CenterArs SyntheticaiGEM – The International Genetically Engineered Machine competitionSome synthetic biology companiesPaper watch: Synthetic biologyBuilding blocks for novel...
Facts and figuresAgriculture and green biotechnology in KenyaJomo Kenyatta University of Agriculture and TechnologyRed biotechnology in Kenya
Electroporation is a simple and versatile approach for DNA transfer but needs to be optimized for specific cells. We conducted square wave electroporation experiments for rat dental follicle cells under various conditions. These experiments indicated that the optimal electroporation electric...
Hemoglobin is a member of the hemoprotein superfamily whose main role is to transport O2 in vertebrate organisms. It has two known promiscuous enzymatic activities, peroxidase and oxygenase. Here we show for the first time that bovine hemoglobin also presents a catalase‐like activity...
The study of the non‐viral gene delivery process at the molecular level, e.g. during the transfection of mammalian cells, is currently limited by the difficulties of specifically detecting the transfected plasmid DNA within the cells. Here we describe the in vivo production of...
Transaminases catalyze amino transfer reactions from amino donors such as amino acids or amines to keto acids or ketones to give chiral amino acid or amines in optically pure form. ॅ‐Amino acid dehydrogenases catalyze the asymmetric reductive amination of ॅ‐keto acids using ammonia as...
Synthetic biology – computational methodsKaznessis et al., Biotechnol. J. 2009, 4, 1392–1405Professor Yiannis Kaznessis from Minnesota (MN, USA) discusses how theoretical synthetic biology may liberate empiricism in biological sciences beyond the unaided human brain. Because synthetic...
To improve butanol selectivity, Clostridium acetobutylicum M5(pIMP1E1AB) was constructed by adhE1‐ctfAB complementation of C. acetobutylicum M5, a derivative strain of C. acetobutylicum ATCC 824, which does not produce solvents due to the lack of megaplasmid pSOL1. The gene products of...
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