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One of the challenges in producing a PEGylated therapeutic protein is that the PEGylation reaction typically generates a mixture of both singly and multiply PEGylated species. The objective of this study was to examine the feasibility of using ultrafiltration for the purification of a singly...
A novel transcriptional regulator responding to glucose was designed with a substrate‐binding protein (SBP) as a probe towards intracellular sensing system for glucose in mammalian cells. A chimeric protein of an SBP for glucose (GBP) and a LacI‐type regulator, LacI (SLCPGL), was designed,...
Flat‐sheet membrane discs represent the current standard format used for experimental prediction of the scale‐up of normal flow filtration processes. Use of this format is problematic, however, since the scale‐down results typically show a 40–55% difference in performance compared to...
In this work, a novel strategy for the controlled fabrication of biomolecular stimulus responsive water‐in‐oil‐in‐water (W/O/W) multiple emulsion using the membrane emulsification process was investigated. The emulsions interface was functionalized with a biomolecule able to function as a...
Due to its availability, low‐price, and high degree of reduction, glycerol has become an attractive carbon source for the production of fuels and reduced chemicals. Using the platform we have established from the identification of key pathways mediating fermentative metabolism of glycerol, this...
Pure culture biofilms of the ammonia‐oxidizing bacterium Nitrosomonas europaea were grown in a Drip Flow Biofilm Reactor and exposed to the aromatic hydrocarbons phenol and toluene. Ammonia oxidation rates, as measured by nitrite production in the biofilms, were inhibited 50% when exposed to 56...
Recently, the prospect of using Escherichia coli as a host for human glycoprotein production has increased due to detailed characterization of the prokaryotic N‐glycosylation process and the ability to transfer the system into this bacterium. Although functionality of the native Campylobacter...
We have previously reported the development of a 100% genetically defined engineered Escherichia coli strain capable of producing L‐valine from glucose with a high yield of 0.38 g L‐valine per gram glucose (0.58 mol L‐valine per mol glucose) by batch culture. Here we report a systems...
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