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We have previously demonstrated that baculovirus can efficiently transduce human mesenchymal stem cells (MSCs) and MSCs‐derived adipogenic, chondrogenic, and osteogenic progenitors without compromising the differentiation capacity. Remarkably, the transgene expression level and duration varied...
Entropic interaction chromatography (EIC) provides efficient size‐based separation of protein mixtures through the entropy change associated with solute partitioning into a layer of hydrophilic homopolymer that has been end‐grafted within the pores of a macroporous chromatography support. In...
The biotransformation of toluene to 3‐methycatechol (3MC) via Pseudomonas putida MC2 was used as a model system for the development of a biphasic process offering enhanced overall volumetric productivity. Three factors were investigated for the identification of an appropriate organic solvent...
Insoluble protein particles showing high specific enzyme activity are potentially useful biocatalysts. The commercialized crosslinked enzyme crystals and aggregates have the disadvantage that their preparation requires isolation of the protein before the critical precipitation step. We introduce...
A tailor‐made apparatus called ammoniometer, which is a batch mode respirometer applied to the study of ammonia biodegradation in biofilter media, has been used to evaluate adsorption, absorption, and biodegradation in five different organic materials (compost, coconut fibre, bark, pruning...
The ability to regulate apoptosis in mammalian cell cultures represents one approach to developing more economical and efficient processes. Genetic modification of cells using anti‐apoptotic genes is one method that may be used to improve cellular performance. This study investigates a method...
The purpose of this work is to determine the cause of the cyclization of the N‐terminal glutamine in recombinant proteins and monoclonal antibodies. This cyclization reaction commonly occurs on the N‐terminal of light and/or heavy chains of antibodies and leads to heterogeneity of the final...
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