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A new hydrophobic and catalytic membrane was prepared by immobilizing Penicillin G acylase (PGA, EC.188.8.131.52) from E. coli on a nylon membrane, chemically grafted with butylmethacrylate (BMA). Hexamethylenediamine (HMDA) and glutaraldehyde (Glu) were used as a spacer and coupling agent,...
Calcium alginate beads were thermally treated at temperatures ranging from 25°C to 130°C for periods of up to 30 minutes. Important modifications to the structure of the alginate beads were shown to be a function of the temperature and period of incubation at each temperature. Modifications to...
Although protein fouling is a critical factor governing the performance of microfiltration systems, there have been relatively few studies comparing the fouling behavior of different proteins. Flux‐decline data were obtained for the filtration of bovine serum albumin, lysozyme, pepsin,...
We introduce a robust and scale‐flexible approach to macromolecule purification employing tailor‐made magnetic adsorbents and high‐gradient magnetic separation technology adapted from the mineral processing industries. Detailed procedures for the synthesis of large quantities of low‐cost...
A cascade of two enzymatic transformations is employed in a one‐pot synthesis of cephalexin. The nitrile hydratase (from R. rhodochrous MAWE)‐catalyzed hydration of D‐phenylglycine nitrile to the corresponding amide was combined with the penicillin G acylase (penicillin amidohydrolase, E.C....
The high‐pressure freezing (HPF) technique was applied to the cryo‐immobilization of alginate gels and the quality of the freezing analyzed on a TEM by comparison of the segregation pattern of samples of decreasing thickness. Dynamic simulations of heat transfer within an idealized slab of...
Poly(N‐isopropylacrylamide), or PNIPAAm, is considered a “smart” polymer because it sharply precipitates when heated above a critical temperature, about 32°C in water, and redissolves when cooled. Conjugates made of PNIPAAm and IgG antibodies also exhibit the same critical temperature...
Lipolysis of butter oil in a hollow fiber reactor containing an immobilized calf pregastric esterase was studied at 40°C, a pH of 6.0, and glycerol concentrations of 0, 150, and 500 g/L in the buffer solution. The concentrations of 10 fatty acid species in the lipolyzed product were determined...
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