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Continuous mammalian cell lines are important hosts for the production of biological pharmaceuticals. However, these cell lines show some severe disorders in primary metabolism, which they have in common with many cancer cells. This leads to a high throughput of substrates giving a low energy...
Tissue‐engineered pancreatic constructs based on immunoisolated, insulin‐secreting cells are promising in providing an effective, relatively inexpensive, long‐term treatment for type I (insulin‐dependent) diabetes. An in vitro characterization of construct function under conditions...
A new family of polymer conjugates is proposed to overcome constraints in the applicability of aqueous two‐phase systems for the recovery of lactic acid. Polyethylene glycol–polyethylenimine (PEI) conjugates and ethylene oxide propylene oxide–PEI (EOPO‐PEI) conjugates were synthesized....
Hemicellulose residues can be hydrolyzed into a sugar syrup using dilute mineral acids. Although this syrup represents a potential feedstock for biofuel production, toxic compounds generated during hydrolysis limit microbial metabolism. Escherichia coli LY01, an ethanologenic biocatalyst...
A simple biofilm model was developed to simulate the competition between two microorganisms for a common inhibitory substrate. The following assumptions were made for the simulations: (1) the biofilm has a uniform thickness and is composed of 5 segments, (2) growth of two microorganisms A and B...
In this study we show that proteins can be partitioned and separated in a novel aqueous two‐phase system composed of only one polymer in water solution. This system represents an attractive alternative to traditional two‐phase systems which uses either two polymers (e.g., PEG/dextran) or one...
Bioartificial pancreatic constructs based on immunoisolated, insulin‐secreting cells have the potential for providing effective, long‐term treatment of type I (insulin‐dependent) diabetes. Use of insulinoma cells, which can be amplified in culture, relaxes the tissue availability limitation...
Subtilisin Carlsberg, an alkaline protease from Bacillus licheniformis, was modified with polyoxyethylene (PEG) or aerosol‐OT (AOT), and the solubility, conformation, and catalytic activity of the modified subtilisins in some organic media were compared under the same conditions. The solubility...
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