Within the last year
Within the past 3 years
1 - 6 of 6 articles
The synthesis of a proteolytically unstable protein, originally designed for periplasmic export in recombinant Escherichia coli BL21(DE3), a strain naturally deficient for the ATP‐dependent protease Lon (or La) and the outer membrane protease OmpT, is associated with a severe growth inhibition....
Pyruvate decarboxylase is a key enzyme in the production of low‐molecular‐weight byproducts (ethanol, acetate) in biomass‐directed applications of Saccharomyces cerevisiae. To investigate whether decreased expression levels of pyruvate decarboxylase can reduce byproduct formation, the PDC2...
Nonlinear kinetics are commonly observed in the enzymatic hydrolysis of cellulose. This nonlinearity could be explained by any or all of the following three factors: enzyme inactivation, product inhibition, or substrate heterogeneity. In this study, four different approaches were applied to test...
The enzyme cellulase, a multienzyme complex made up of several proteins, catalyzes the conversion of cellulose to glucose in an enzymatic hydrolysis‐based biomass‐to‐ethanol process. Production of cellulase enzyme proteins in large quantities using the fungus Trichoderma reesei requires...
This review analyzes the role of cutinases in nature and their potential biotechnological applications. The cloning and expression of a fungal cutinase, Fusarium solani f. pisi, in Escherichia coli and Saccharomyces cerevisiae hosts are described. The three‐dimensional structure of this...
The synergistic interaction among three β‐specific glycosidases from the hyperthermophilic archaeon Pyrococcus furiosus, namely two endoglucanases (EglA and LamA) and an exo‐acting β‐glucosidase (Bgl), on barley‐glucan and laminarin, was examined. In addition to following glucose...
Read and print from thousands of top scholarly journals.
Sign up with Facebook
Sign up with Google
Already have an account? Log in
Save this article to read later. You can see your Read Later on your DeepDyve homepage.
To save an article, log in first, or sign up for a DeepDyve account if you don’t already have one.
Sign Up Log In
To subscribe to email alerts, please log in first, or sign up for a DeepDyve account if you don’t already have one.
To get new article updates from a journal on your personalized homepage, please log in first, or sign up for a DeepDyve account if you don’t already have one.