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10.1002/bit.260480306.abs On‐line, “real‐time” monitoring of product concentration is important for mammalian cell culture fermentation. The continuous measurement of monoclonal antibodies allows for instantaneous determination of cell productivity and effective manipulation of the...
10.1002/bit.260480315.abs Steady state metabolic parameters for hybridoma cell line H22 were determined over a wide range of cell densities and specific growth rates in a filtration based homogeneous perfusion reactor. Operating the reactor at perfusion rates of 0.75, 2.0, and 2.9 day−1(each at...
10.1002/bit.260480313.abs The influence of two mixing geometries (at the same scale) with different flow energy distributions on the performance of the gibberellic acid fermentation and on the morphology of the producing fungus Fusarium moniliforme was investigated. Fermentations were performed...
10.1002/bit.260480309.abs The biological phosphorus removal process is a process which depends basically on three internal storage compounds. Poly‐β‐hydroxybutyrate (PHB) produced during the anaerobic phase is used as substrate for biomass, polyphosphate, and glycogen formation. The reaction...
10.1002/bit.260480303.abs Kinetics of lipase‐catalyzed hydrolysis of esters were modeled using reactant activities for aqueous‐organic, biphasic systems. By using thermodynamic activities of the substrates in ordinary rate equations, the kinetic parameters were corrected for the contribution...
10.1002/bit.260480308.abs Biosurfactants were prepared by enzymatic esterification of sugars and sugar alcohols in nonaqueous media. Sorbitol monooleate was produced in pure molten substrates, with reduced pressure to remove water. The results were compared to synthesis in organic solvent, with...
10.1002/bit.260480310.abs A metabolic model of the biological phosphorus removal process has been developed and validated previously for complex conversions during the process under anaerobic and aerobic conditions at different growth rates in sequencing batch reactors in steady state. For...
10.1002/bit.260480304.abs Humicola lanuginosa lipase (HIL) and Rhizomucor miehei lipase (RrnL), isolated from commercial preparations of Lipolase and Lipozyme, respectively, were solubilized in AOT‐stabilized water‐in‐oil (w/o) microemulsions in n‐heptane and aspects of their hydrolysis...
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