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10.1002/bit.260480110.abs Bacterial cell surface layers (S‐layers) which show a crystalline structure, defined pores, and a regular arrangement of functioal groups can be used for production of isoporous ultrafiltration membranes and as a matrix for immobilization of macromolecules....
10.1002/bit.260480106.abs The fluorescent probe PO‐PRO‐3 was investigated as a potential ligand for the affinity immobilization and purification of genomic or plasmid DNA fragments. Affinities and mechanisms for PO‐PRO‐3 binding to superhelical and linearized pUC 18 plasmid DNA were...
10.1002/bit.260480111.abs Lipolase and Lipozyme are produced in large quantities (as a result of genetic engineering and overexpression) for the detergents market and provide a cheap source of highly active biocatalysts. Humicola lanuginosa lipase (HIL) and Rhizomucor miehei lipase (RmL) have...
10.1002/bit.260480104.abs Disaccharides were microbaially transformed to their corresponding 3‐keto‐derivatives by resting cells of Agrobacterium tumefaciens NCPPB 396. The kinetics and yield of this highly specific oxidation depend on several factors. The oxygen concentration especially has...
10.1002/bit.260480108.abs Crosslinked polystyrene resins containing a low level of either basic or acidic groups were used for supports of α‐chymotrypsin (CT), which catalyzed the transesterification of N‐acetyl‐L‐phenylalanine ethyl ester (AcPheOEt) with propanol in toluene. With a...
10.1002/bit.260480103.abs Although filamentous microorganisms are widely used in industrial fermentation processes, their growth and differentation are not yet fully understood, because their biomass is structured, and therefore difficult to descrbie and to quantify. This lack of appropriate...
10.1002/bit.260480105.abs A simple method for preparing multicellular spheroids from varied cell types has been successfully developed by using a stepwise gradient surface in cell attachability or detachability. The surface was composed of poly‐N‐isopropylacrylamide (PNIPAAm), a temperature...
10.1002/bit.260480109.abs The Model presented in this work demonstrates the combination of cell‐cycle model with a model describing the growth and conversion kinetics of hybridoma cells in a steady‐state continuous culture. The cell‐cycle model is based upon a population balance model as...
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