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10.1002/bit.260360814.abs Simultaneous determination of cell size and DNA content of hybridomas (HB‐32) revealed a direct correlation between average cell volume and progression through the cell cycle. Pseudocontinuous experiments showed that G1 cells, as estimated from cell size measurements,...
10.1002/bit.260360811.abs In Part II, the process utility of a thermotolerant methylotrophic bacterium is evaluated with respect to its dynamic response when various substrate or nutrient pulses are imposed on it while growing under steady‐state conditions in a chemostat. The pulses...
10.1002/bit.260360815.abs To attain both high productivity and efficient recovery of ethanol from broth, a membrane bioreactor consisting of a jar fermentor and a pervaporation system was applied to the direct production of ethanol from uncooked starch with a thermophilic anaerobic bacterium,...
10.1002/bit.260360803.abs The fluid dynamic design and characterization of a low mechanical stress agitator and bioreactor vessel for large‐scale bioprocessing using anchorage dependent mammalian cells is considered. The complex and fragile nature of mammalian cells puts stringent constraints...
10.1002/bit.260360805.abs When a mechanism is used to determine possible deactivation kinetics, a certain consistency with the kinetics of the main reaction is found. This result is examined in the light of experimental evidence obtained with bovine liver and Aspergillus catalases.
10.1002/bit.260360812.abs Microporous‐membrane‐based extractive product recovery in product‐inhibited fermentations allows in situ recovery of inhibitory products in a nondispersive fashion. A tubular bioreactor with continuous strands of hydrophobic microporous hollow fibers having...
10.1002/bit.260360807.abs The growth of two lymphocyte cell lines, a hybridoma cell line and a human cutaneous T cell lymphoma (HuT78), was studied in fed‐batch culture, and unstructured models of growth developed. A criteria was established to insure that the growth rate varied by less than a...
10.1002/bit.260360816.abs Even when microorganisms are grown in highly agitated fermentors, calculation predicts a mismatch between the microscale of the turbulence (where the smallest eddy is typically 50–300 μm diameter) and the cellular dimensions (1–5 μm). The cell thus spends...
10.1002/bit.260360809.abs A polyethylene‐g‐acrylic acid (PE‐g‐AA) graft copolymer was prepared via gamma‐ray‐irradiation‐induced postirradiation procedures, and was used as support material for the immobilization of glucose oxidase. Soluble carbodiimides were used as the coupling...
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